{"id":14162,"date":"2024-07-23T09:11:39","date_gmt":"2024-07-23T09:11:39","guid":{"rendered":"https:\/\/www.drknowhk.org\/?p=14162"},"modified":"2024-08-26T09:27:06","modified_gmt":"2024-08-26T09:27:06","slug":"sars-cov-2-spike-does-not-interact-with-the-t-cell-receptor-or-directly-activate-t-cells","status":"publish","type":"post","link":"https:\/\/www.drknowhk.org\/mo\/sars-cov-2-spike-does-not-interact-with-the-t-cell-receptor-or-directly-activate-t-cells\/","title":{"rendered":"SARS-CoV- 2 spike does not interact with the T cell receptor or directly activate T cells"},"content":{"rendered":"<p class=\"qtranxs-available-languages-message qtranxs-available-languages-message-mo\">\u5c0d\u4e0d\u8d77\uff0c\u6b64\u5185\u5bb9\u53ea\u9069\u7528\u65bc<a href=\"https:\/\/www.drknowhk.org\/en\/wp-json\/wp\/v2\/posts\/14162\" class=\"qtranxs-available-language-link qtranxs-available-language-link-en\" title=\"English\">English<\/a>\u3002<\/p><p>By <span class=\"authors\"><span role=\"list\"><span role=\"listitem\">Stephanie A. Gaglione<\/span><\/span><\/span> et al.<\/p>\n<p>Source PNAS<\/p>\n<section id=\"abstract\" role=\"doc-abstract\">\n<h2>Abstract<\/h2>\n<div role=\"paragraph\">SARS-CoV-2 infection can induce multisystem inflammatory syndrome in children, which resembles superantigen-induced toxic shock syndrome. Recent work has suggested that the SARS-CoV-2 spike (S) protein could act as a superantigen by binding T cell receptors (TCRs) and inducing broad antigen-independent T cell responses. Structure-based computational modeling identified potential TCR-binding sites near the S receptor-binding domain, in addition to a site with homology to known neurotoxins. We experimentally examined the mechanism underpinning this theory\u2014the direct interaction between the TCR and S protein. Surface plasmon resonance of recombinantly expressed S protein and TCR revealed no detectable binding. Orthogonally, we pseudotyped lentiviruses with SARS-CoV-2 S in both wild-type and prefusion-stabilized forms, demonstrated their functionality in a cell line assay, and observed no transduction, activation, or stimulation of proliferation of CD8<sup>+<\/sup> T cells. We conclude that it is unlikely that the SARS-CoV-2 spike protein engages nonspecifically with TCRs or has superantigenic character.<\/div>\n<div role=\"paragraph\"><\/div>\n<div role=\"paragraph\">Read more<a href=\"https:\/\/www.drknowhk.org\/wp-content\/uploads\/2024\/08\/gaglione-et-al-2024-sars-cov-2-spike-does-not-interact-with-the-t-cell-receptor-or-directly-activate-t-cells.pdf\"> click here<\/a><\/div>\n<\/section>\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>\u5c0d\u4e0d\u8d77\uff0c\u6b64\u5185\u5bb9\u53ea\u9069\u7528\u65bcEnglish\u3002By Stephanie A. Gaglione et al. Source PNAS Abstract SARS-CoV-2 infection can induce multisystem inflammatory syndrome in children, which resembles superantigen-induced toxic shock syndrome. Recent work has suggested that the SARS-CoV-2 spike (S) protein could act as a superantigen by binding T cell receptors (TCRs) and inducing broad antigen-independent T cell responses. Structure-based computational modeling [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":14164,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[21,52],"tags":[],"class_list":["post-14162","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-scientific-reports","category-virology-pathophysiology"],"_links":{"self":[{"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/posts\/14162","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/comments?post=14162"}],"version-history":[{"count":1,"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/posts\/14162\/revisions"}],"predecessor-version":[{"id":14165,"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/posts\/14162\/revisions\/14165"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/media\/14164"}],"wp:attachment":[{"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/media?parent=14162"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/categories?post=14162"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.drknowhk.org\/mo\/wp-json\/wp\/v2\/tags?post=14162"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}